1. Protective Effects of Hydrogen Inhalation on Rat Model of Chronic Obstructive Pulmonary Disease.

■Journal
Progress in Modern Biomedicine
■Year
2015
■Instute
-
■Authors
Wenye Geng, Ming Fan, Jingxi Zhang
■Diseases
COPD
■Dose
67% H2 / 33% O2 Mixture
■Rate
1h / bid Total 2 weeks
■Observations
Lung
■Effects
Inhalation of high-concentrated H2 can significantly reduce the oxidative stress and inflammation level in rats' blood and lung tissues caused by smoking, improve the pathological changes of lung tissue, enhance the lung function of animals, and be an effective adjuvant therapy of COPD.

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2. Therapeutical effects of hydrogen inhalation on rat model of bronchial asthma.

■Journal
International Journal of Respiration
■Year
2015
■Instute
-
■Authors
Ning Zhang, Jingxi Zhang
■Diseases
Bronchial asthma
■Dose
67% H2 / 33% O2 Mixture
■Rate
1h / bid Total 2 weeks
■Observations
Lung
■Effects
Compared with the control, inhalation of high-concentrated H2 can effectively alleviate the pathological changes of lung tissue. It also has adjuvant effect on the rat asthma induced by egg protein, with a possible mechanism of hydrogen's antioxidant effect.

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3. Inhalation of hydrogen gas ameliorates glyoxylate-induced calcium oxalate deposition and renal oxidative stress in mice.

■Journal
International Journal of Clinical and Experimental Pathology
■Year
2015
■Instute
-
■Authors
Zhongjiang Peng, Wei Zhan, Zhiyong Guo
■Diseases
Kidney stone
■Dose
67% H2 / 33% O2 Mixture
■Rate
30 min / per time Total 1 week
■Observations
Kidney
■Effects
Inhalation of H2 can reduce the formation of calcium oxalate crystals, reduce oxidative stress and inflammation in the kidney. H2 may be a candidate for prevention of kidney stones without adverse reaction

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4. Inhalation of water electrolysis-derived hydrogen ameliorates ischiemia-reperfusion injury in rats.

■Journal
Neuroscience
■Year
2016
■Instute
-
■Authors
Jin Cui, Xiao Chen, Jiacan Su, et al.
■Diseases
Cerebral ischemia reperfusion injury
■Dose
67% H2 / 33% O2 Mixture
■Rate
inhaled immediately after reperfusion，2h
■Observations
Brain, liver, kidney
■Effects
Inhalation of high-concentrated H2 prepared by electrolyzed water was not seen any damage to brain, liver and kidney of rats, and it had protective effect on cerebral ischemia reperfusion injury in rats. Thus, high-concentrated H2 could improve oxidative stress in human body.

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5. Protective effect of hydrogen on the lung of sanitation workers exposed to haze.

■Journal
Chinese Journal of Tuberculosis Respiration
■Year
2016
■Instute
-
■Authors
Zhijing Gong, Jitao Guan, Xixing Yan
■Diseases
Haze
■Dose
67% H2 / 33% O2 Mixture
■Rate
1h/d Total 30 days
■Observations
Lung
■Effects
Inhalation of H2 can help to reduce the inflammatory level associated with oxidative stress injury in the airway, and may also have a certain effect on inhibiting systemic inflammatory response, while improving respiratory symptoms such as cough in sanitation workers.

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6. Postconditioning with inhaled hydrogen promotes survival of retinal ganglion cells in a rat model of retinal ischemia/reperfusion injury.

■Journal
Brain Research
■Year
2016
■Instute
-
■Authors
Ruobing Wang, Jiangchun Wu, Lin Liu, et al.
■Diseases
Retinal ischemia reperfusion injury
■Dose
67% H2 / 33% O2 Mixture
■Rate
1h/d Total 1 week
■Observations
Retinal
■Effects
Inhalation of high-concentrated H2 can protect the RGC damage caused by retinal i/r, and its mechanism may be related to antioxidant, anti-inflammatory and anti-apoptosis.

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7. Effects of inhaling high-concentrated H2 on neuronal apoptosis and apoptosis-related protein expression in cerebral cortex after focal cerebral ischemia-reperfusion injury in rats.

■Journal
Guangdong Medical Journal
■Year
2016
■Instute
-
■Authors
Yuning Xia, Xuemei Li, Yongxing Tan
■Diseases
Cerebral ischemia and reperfusion injury
■Dose
67% H2 / 33% O2 Mixture
■Rate
2 hours
■Observations
Brain
■Effects
Reperfusion along with the inhalation of high-concentrated H2 can inhibit neuronal apoptosis by increasing the inhibition of apoptosis protein Bcl-2 and lowering the expression of apoptosis-inducing protein bax, significantly reducing the area of cerebral infarction in rats. Protective effect on brain I/R injury in rats.

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8. Effects of Hydrogen Gas Inhalation on Endometriosis in Rats.

■Journal
Reproductive Sciences
■Year
2016
■Instute
-
■Authors
He Yan, Shi Jiazi, Liu Yuhuan,et al.
■Diseases
Endometriosis
■Dose
67% H2 / 33% O2 Mixture
■Rate
1h/d Total 4 weeks
■Observations
Endometrium
■Effects
The result showed: inhalation of H2 (67%) and O2 (33%) mixed gas is beneficial to the endometriosis in rat model with clinical effect.

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9. Inhalation of H2 slows the development of COPD lung disease induced by smoking in rat model.

■Journal
International Journal of COPD
■Year
2017
■Instute
-
■Authors
Xiaoyu liu, Cuiqing Ma,Baojian Xue,et al.
■Diseases
COPD
■Dose
2%、22%、41.6%
■Rate
1h/bid Total 4 months
■Observations
Lung
■Effects
H2 is expected to be a newly effective treatment for COPD in the future. This study reflects the dose effect of hydrogen, but the effect of COPD inhalation of high-concentrated H2 on oxidative stress and signaling pathway is still to be studied in depth.

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10. High-concentration hydrogen protects mouse heart against ischemia/reperfusion injury through activation of the PI3K/Akt1 pathway.

■Journal
Scientific Reports
■Year
2017
■Instute
-
■Authors
Ouyang Chen,Zhiyong Cao,Xuejun Sun,et al.
■Diseases
Myocardial ischemia-reperfusion
■Dose
67% H2 / 33% O2 Mixture
■Rate
90 min / each time
■Observations
Heart
■Effects
Inhalation of H2 helps the initiation of PI3K-Akt channel, inhibits apoptosis of cardiac cells, and maintains cardiac function.

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11. Molecular hydrogen increases resilience to stress in mice.

■Journal
Scientific Reports
■Year
2017
■Instute
-
■Authors
Qiang Gao, Han Song, Hai-Shui Shi,et al.
■Diseases
Depression, anxiety
■Dose
67% H2 / 33% O2 Mixture
■Rate
3 h / each time
■Observations
Hippocampus and blood
■Effects
This study was aimed at the effect of stress resilience in rats. The result showed that hydrogen molecules could be used as a new strategy to prevent the occurrence of stress-related diseases.

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12. Inhalation of high-concentrated H2 improves hepatic ischemia reperfusion injury through A2A receptor-mediated pi3k-akt pathway.

■Journal
Biochemical Pharmacology
■Year
2017
■Instute
-
■Authors
He Li, Ouyang Chen, Xuejun Sun,et al.
■Diseases
Hepatic ischemia reperfusion injury
■Dose
67% H2 / 33% O2 Mixture
■Rate
1h/per time
■Observations
Liver
■Effects
Inhalation of H2 helps to activate PI3K-Akt channel and indirectly inhibits apoptosis of liver cells to maintain liver function.

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13.
Hydrogen gas inhibits lung cancer progression through targeting SMC3.

■Journal
Biomedicine & Pharmacotherapy
■Year
2018
■Instute
-
■Authors
Dongchang Wang, Lifei Wang, Gang Chen, et al.
■DiseasesLung cancer cell line
■Dose
67% H2 / 33% O2 Mixture
■Rate
2h/d for 1 month
■Observations
A549 and H1975 cancer cell lines
■Effects
H2 can inhibit the growth, migration and invasion of cancer cells, and catalyze the apoptosis of cells. H2 induced A549 and H1975 remain in the g2/m phase of the cell cycle; In addition, H2 lowered NIBPL, SMC3, SMC5 and SMC6 expression ... All data showed that H2 can inhibit the progression of lung cancer and regulate SMC3, which provides a new method for the treatment of lung cancer.

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14. Effect and mechanism of hydrogen suppressing non-small cell lung cancer.

■Journal
International Journal of Respiration
■Year
2018
■Instute
-
■Authors
Dongchang Wang, Yunxia Zhaou, Gang Chen
■Diseases
Non-small cell lung cancer
■Dose
67% H2 / 33% O2 Mixture
■Rate
4 Weeks
■Observations
Tumor
■Effects
Inhalation of high-concentrated H2 may inhibite the growth of non-small lung cancer, which mechanism is related with the inhibition of the expression of Ki-67, COX-2, VEGF.

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15. Hydrogen gas inhalation protects against cigarette smoke-induced COPD development in mice.

■Journal
Journal of Thoracic Disease
■Year
2018
■Instute
-
■Authors
Wenju Lu，Defu Li，Zeguang Zheng,et al.
■Diseases
COPD
■Dose
42% H2 Concentration(Volume ratio)(Animal Exp.)(Cell Culture)
■Rate
1h/bid for every 6 - 8 hours(Animal Exp.)Last 24 hours(Cell Culture)
■Observations
16 HBE cell lines
■Effects
H2 can inhibit the COPD development induced by smoking in the body of rat, slow down lung function, improve emphysema, inflammatory cell infiltration and other situations.

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16. Inhalation of Hydrogen of Different Concentrations Ameliorates Spinal Cord Injury in Mice by Protecting Spinal Cord Neurons from Apoptosis, Oxidative Injury and Mitochondrial Structure Damages

■Journal
Cell PhysiolBiochem
■Year
2018
■Instute
-
■Authors
Xiao Chen, Jin Cui ，Jiacan Su,et al.
■Diseases
Spinal cord injury
■Dose
H2 Concentration: 50%, 65%, 75%(Volume ratio) (Cell culture);H2 Concentration: 75%(Volume ratio) (Animal Exp.);
■Rate
1h/d for 1 week
■Observations
Spinal cord neurons
■Effects
The in-vitro experiment constructed the mechanical injury model of spinal cord neurons, and the morphology of neurons was improved after given high-concentrated H2. Oxidative stress injury, apoptosis and mitochondrial damage were inhibited significantly; in-vivo experiment, C57 the spinal cord clamp injury model in mice, further verified the protective effect of high-concentrated H2 on spinal cord injury.

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17. Hydrogen gas reduces chronic intermittent hypoxia‐induced hypertension by inhibiting sympathetic nerve activity and increasing vasodilator responses via the antioxidation.
■Journal
Journal of Cellular Biochemistry
■Year
2018
■Instute
-
■Authors
Peng Guan, Xiao‐Meng Lin, En-Sheng Ji,et al.
■Diseases
Chronic Intermittent Hypoxia (CIH)
■Dose
67% H2 / 33% O2 Mixture
■Rate
2 hours before the exposure of intermittent hypoxia, total 35 days
■Observations
Brain
■Effects
Mechanistically, inhibition of sympathetic activity and reduction of systemic vascular resistance may participate in this process via the antioxidant activity of H2.

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18. Hydrogen ameliorates chronic intermittent hypoxia-induced neurocognitive impairment via inhibiting oxidative stress.

■Journal
Brain Research Bulletin
■Year
2018
■Instute
-
■Authors
Wenya Li, Shengchang Yang, En-sheng Ji,et al.
■Diseases
Chronic Intermittent Hypoxia (CIH)
■Dose
67% H2 / 33% O2 Mixture
■Rate
2 hours before the exposure of intermittent hypoxia, total 35 days
■Observations
Brain
■Effects
Inhaled H2 can reduce the speed of chronic intermittent hypoxia-induced neurological impairment by anti-oxidative and anti-apoptotic effects. These findings may be a kind of supportive therapy for obstructive sleep apnea.

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19. Improvement of hydrogen on liver oxidative stress injury in chronic intermittent hypoxia rats.

■Journal
Chinese Journal of Applied Physiology
■Year
2018
■Instute
-
■Authors
Shengchang Yang, Lingling Chen, Ensheng Ji et al.
■Diseases
Chronic Intermittent Hypoxia (CIH)
■Dose
67% H2 / 33% O2 Mixture
■Rate
1 hour before the exposure, total 35 days
■Observations
Liver
■Effects
Inhalation of high-concentrated H2 can significantly improve the effects of intermittent hypoxia on liver damage in rats, primarily through alleviating oxidative stress injury and improving liver cell damage.

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20. Breathing Hydrogen-Oxygen Mixture Decreases Inspiratory Effort in Patients with Tracheal Stenosis.

■Journal
Respiration
■Year
2018
■Instute
-
■Authors
Zi-Qing Zhou, Chang-Hao Zhong, Shi-Yue Li, et al.
■Diseases
Airway stenosis
■Dose
67% H2 / 33% O2 Mixture
■Rate
67% H2,inhalation 2 hours before operation
■Observations
Diaphragmatic muscle
■Effects
Inhalation of high-concentrated H2 can quickly reduce airway resistance in patients with tracheal stenosis, and will not change respiration, heart rate, blood pressure and pulse, blood oxygen and other parameters; no adverse reaction was observed.

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21. Effects of Post-Treatment Hydrogen Gas Inhalation on Uveitis Induced by Endotoxin in Rats.

■Journal
Medical Science Monitor
■Year
2018
■Instute
-
■Authors
Weiming Yan, Tao Chen, Zuoming Zhang,et al.
■Diseases
Uveitis
■Dose
67% H2 / 33% O2 Mixture
■Rate
Total 96 hours
■Observations
Microglial cell
■Effects
Inhalation of H2 inhibits the rise of water-resistant proteins (AQH) and reduces activation of microglial cells.

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22. Effect of High Concentration of Hydrogen Gas Inhalation on Postoperative Delirium in Elderly Patients Receiving Hip Fracture Surgery.

■Journal
Journal of Clinical Anesthesiology
■Year
2018
■Instute
-
■Authors
Gang Liu, Yonghao Yu, Keliang Xie
■Diseases
Postoperative delirium
■Dose
67% H2 / 33% O2 Mixture
■Rate
67% H2 inhalation in 1 hour before and after operation
■Observations
Blood
■Effects
Inhalation of H2 can significantly reduce the incidence of postoperative delirium in elderly patients with hip fractures.

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